By Tanya E. S. Dahms, Kirk J. Czymmek
The objective of this quantity is to explain the newest advances in microscopic equipment, together with built-in recommendations, as utilized to mycology. each one bankruptcy will offer a short review of a specific microscopic procedure with linked merits and barriers, the examine questions that may be safely addressed utilizing those microscopic equipment, the way it has been effectively utilized to handle mycological examine questions, together with helping and complimentary concepts, and which destiny questions may be addressed.
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Extra info for Advanced Microscopy in Mycology
6 PALM analysis of the membrane-associated polarity marker TeaR in A. nidulans. a, b The size of TeaR domains imaged by widefield epifluorescent microscopy is approximately 440 nm (upper, green line), whereas PALM revealed that the size is actually closer to 100 nm ( lower, red line, PALM image is constructed by 1000 frames, time resolution 50 ms). c The membrane domains were defined by cluster analysis, setting the threshold on the number of neighboring molecules (ten molecules) within a certain distance r (50 nm).
Fungal Genet Biol 44:1011–1023 Day RD, Davidson MW (2009) The fluorescent protein palette: tools for cellular imaging. Chem Soc Rev 38:2887–2921. 1039/B901966A Delgado-Álvarez DL, Callejas-Negrete OA, Gómez N, Freitag M, Roberson RW, Smith LG, Mouriño-Pérez RR (2010) Visualization of F-actin localization and dynamics with live cell markers in Neurospora crassa. Fungal Genet Biol 47:573–586 Delgado-Álvarez DL, Bartnicki-García S, Seiler S, Mouriño-Pérez RR (2014) Septum development in Neurospora crassa: the Septal Actomyosin Tangle.
Eukaryot Cell 6(9):1521–1527. (Available from PM: 17660363) Espeso EA, Osmani SA (2008) Nuclear pore complex and transport in Aspergillus nidulans. In: Goldman GH, Osmani SA (eds) The Aspergilli: genomics, medical aspects, biotechnology, and research methods. Taylor & Francis Group, Boca Raton, pp 261–277 Etxebeste O, Ni M, Garzia A, Kwon NJ, Fischer R, Yu JH, Espeso EA, Ugalde U (2008) Basiczipper-type transcription factor FlbB controls asexual development in Aspergillus nidulans. Eukaryot Cell 7(1):38–48.